New Screening Method Quickly Identifies Mice Bred For Bone Marrow Regeneration Studies

Date: Jun-10-2013Immunocompromised mice, created by inactivating the genes that would allow them to recognize and attack donor cells or organs, are critical for studies of bone marrow reconstitution. A more rapid and reliable technique for identifying these mice in breeding colonies is described in an article in BioResearch Open Access, a peer-reviewed open access journal from Mary Ann Liebert, Inc., publishers. The article is available free on the BioResearch Open Access website.*

Alejandro Ferrer, Adam Schrum, and Diana Gil, College of Medicine, Mayo Clinic (Rochester, MN), designed a simple method for identifying mice with specific gene deletions or replacements, using a DNA amplification technique called polymerase chain reaction, or PCR. They describe the use of this approach in the article "A PCR-Based Method to Genotype Mice Knocked Out for All Four CD3 Subunits, the Standard Recipient Strain for Retrogenic TCR/CD3 Bone Marrow Reconstitution Technology." **

"This technical report describes for the first time a simple PCR-based screen to identify TCR/CD3 knockout mice," says BioResearch Open Access Editor Jane Taylor, PhD, MRC Centre for Regenerative Medicine, University of Edinburgh, Scotland. "This rapid method will provide a valuable tool for all researchers using TCR/CD3 retrogenesis."
Courtesy: Medical News Today Note: Any medical information available in this news section is not intended as a substitute for informed medical advice and you should not take any action before consulting with a health care professional.